Differentiation of hippocampal neuron in cuture

Hippocampal cells from WT embryos were cultured and maintained at 37°C with 5% CO2 inside an incubator. After 1 day, they were placed inside the videomicroscopy platform at 37°C with 5% CO2 (beginning of the film, time=0). Phase-contrast images were taken every 10 minutes to build the film. At time 0 of the film (1 day in culture), there are many neurites (white arrows) with similar lengths. They are highly dynamic, extending and retracting for short distances. One of these neurites became an axon (black