Microscopy: Total Internal Reflection Fluorescence (TIRF) Microscopy (Daniel Axelrod)

Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique is used for, explains the principles of the evanescent wave, gives many examples of different microscope configurations used in TIRFM, and shows how polarized light TIRF can be used to image membrane orientation.