Hatching blastocyst biopsy

In this approach, ZP is opened on D4. On D5 the herniating TE cells are removed for genetic testing. The major disadvantage of this appproach is that in some embryos ICM can enter the opening of ZP and the procedure must be accomplished after a new hole is created. Moreover, asynchronoeus development of the embryos and different time of TE cells herniation requires a sequential timing of the procedure.