Gel Electrophoresis Tips & Tricks

The main causes of bands “smiling” on a gel are: Uneven heating of the gel across different lanes. This is usually caused by high voltage. To avoid this, the user can run the gel slowly (reduce the voltage) so that the temperature inconsistency is minimized. Uneven distribution of the electric field across the gel width. This can be addressed by checking the tank setup for loose contacts or other possible problems with your electrophoresis tank. #NikolaysGeneticsLessons #gelElectrophoresis #gelElectrophoresisExplained #gelElectrophoresisOfDna #gelElectrophoresisProcedure #gelElectrophoresisLab #gelElectrophoresisAnalysis #agaroseGelElectrophoresis #agaroseGelElectrophoresisProtocol #agaroseGelElectrophoresisOfDna #agaroseGelElectrophoresisExplained #dnaGelElectrophoresis #dnaElectrophoresis #electrophoresisOfDna #sdsPageGelElectrophoresis #polyacrylamideGelElectrophoresis