DNA extraction from plants

DNA isolation is a routine procedure to collect DNA for subsequent molecular or forensic analysis. There are three basic and two optional steps in a DNA extraction: Breaking the cells open, commonly referred to as cell disruption or cell lysis, to expose the DNA within. This is commonly achieved by chemical and physical methods-blending, grinding or sonicating the sample. Removing membrane lipids by adding a detergent or surfactants. Removing proteins by adding a protease (optional but almost always done). Removing RNA by adding an RNase (often done). Precipitating the DNA with an alcohol — usually ice-cold ethanol or isopropanol. Since DNA is insoluble in these alcohols, it will aggregate together, giving a pellet upon centrifugation. This step also removes alcohol-soluble salt. See ethanol precipitation. (continue below ↓↓↓)