Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.
Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving. Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.
Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.
#GelElectrophoresis #macromolecules #DNA #RNA #proteins #agarose #pcr #SouthernBlotting #RFLP #DNASequencing #DNAMarker #DNABands #usingAGelElectrophoresisMachine #DNAFingerprinting #howDoesAGelElectrophoresisMachineWork #howDNAMovesThroughGelElectrophoresis #DNALadder #semilogGraph #purposeOfGelElectrophoresis #biotechnology #highSchool #Genetics #electrophoresis #stepsInElectrophoresis #NikolaysGeneticsLessons
1 view
129
29
4 weeks ago 00:05:30 1
ЧТО ТАКОЕ ИНТЕЛЛЕКТ? | IQ
4 weeks ago 00:17:48 1
Nun Shares Stark End Time Scenario: ONLY ONE BILLION WILL REMAIN!
4 weeks ago 00:04:29 1
Gorillaz - Clint Eastwood (Official Video)
4 weeks ago 00:05:30 1
I SEE FIRE - The Hobbit | Low Bass Singer Cover | Geoff Castellucci
4 weeks ago 00:19:45 1
Spitgod - Through Life ’till Death (EP, 2024) 🇵🇹
4 weeks ago 01:06:00 1
I forced TF2 YouTubers to build an unplayable map
4 weeks ago 00:55:04 1
Computer & Technology Basics Course for Absolute Beginners
4 weeks ago 00:02:49 1
Amazing travel and spiritual dance, very nice chill music video
4 weeks ago 00:08:47 1
How to say the time in English
4 weeks ago 00:07:37 1
CRYPTA - From The Ashes (Official Video) | Napalm Records
4 weeks ago 00:19:59 1
Common guitar riffs and licks - the “building blocks of rock“
4 weeks ago 00:00:47 1
The Secret Of Fisherman Fishing Knot Skills How to Tie Fishing Knot Using Tackle #fishing
4 weeks ago 00:41:36 1
TMZ: Diddy’s a Fed, Zuckerberg’s Shirt, Terrence Howard’s Rant & King Charles’ Perennialism!
4 weeks ago 00:13:47 1
12 MIN BOOTY PUMP VOL. 2 - make your butt grow I update of my fave Butt Activation - harder & better
4 weeks ago 00:00:17 1
Optimum Pride Original Meme TikTok (Full)
4 weeks ago 00:09:50 1
Text Logo Reveal Animation in After Effects | After Effects Tutorial
4 weeks ago 01:18:39 1
[무료도안/free pattern] 2편 - 30가지 기초 프랑스자수 30 Basic embroidery
4 weeks ago 00:01:13 1
12 Principles of Animation; 05 Follow Through & Overlapping Action
4 weeks ago 00:07:14 1
How to Microtune Stuff Easily
4 weeks ago 00:00:36 1
Op doorreis? Kies voor onze camping met trekkershut
4 weeks ago 00:24:25 1
SOUL OF DRAGON | Epic Chinese Adventure Orchestral Music | Epic Drum Battle & Flute Mix
4 weeks ago 00:31:31 1
Design, Rig, and Animate a character in Moho - Full Tutorial
4 weeks ago 00:09:45 1
Le coup de pied frontal de Systema par Valentin Talanov !