How to Do a Gram Stain? - Biology Lab Techniques

Gram staining is a method of staining used to distinguish and classify bacterial species into two large groups (gram-positive and gram-negative). The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique. 00:00 - Gram Stain description 00:37 - Heat-fixation 00:53 - Reagents description 01:05 - Crystal violet application 01:30 - Lugol’s reagent application 01:52 - Ethanol/Acetone wash 02:19 - Safranin application 02:41 - Drying 02:44 - Microscopic observation Video produced by Dr Guillaume Erard @guillaumeerard Techniques demonstrated by Ms Ayumu Nishimura Learn more on #microbiology #gramstain #ibbiology